Qiagen RNeasy Mini Kit
For purification of up to 100 µg total RNA from cells, tissues, and yeast
For purification of up to 100 µg total RNA from cells, tissues, and yeast
Technical Specifications :
Feature
|
Specifications
|
Applications
|
Northern, dot,
and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array
analysis, next-generation sequencing
|
Elution volume
|
30–100 µl
|
Format
|
Spin column
|
Main sample type
|
Tissue, cells
|
Processing
|
Manual
(centrifugation)
|
Purification of
total RNA, miRNA, poly A+ mRNA, DNA or protein
|
Total RNA
|
Sample amount
|
0.5–30 mg
|
Technology
|
Silica technology
|
Time per run or
per prep
|
20 minutes
|
Yield
|
Varies
|
The RNeasy Mini Kit allows efficient purification of total
RNA from small amounts of starting material. RNeasy technology simplifies total
RNA isolation by combining the stringency of guanidine-isothiocyanate lysis
with the speed and purity of silica-membrane purification. RNeasy Kits provide
the highest-quality RNA with minimum copurification of DNA.
Procedure
With the RNeasy Mini Kit, total RNA is easily purified from
10 to 1 x 107 animal or human cells, 0.5–30 mg animal or human tissues, and
<5 x 107 yeast cells. Samples are
first lysed and then homogenized. Ethanol is added to the lysate to provide
ideal binding conditions. The lysate is then loaded onto the RNeasy silica
membrane (see figure RNeasy Mini spin column). RNA binds (up to 100 µg
capacity), and all contaminants are efficiently washed away. For certain RNA
applications that are sensitive to very small amounts of DNA, the residual
amounts of DNA remaining can be removed using a convenient on-column DNase
treatment. Pure, concentrated RNA is eluted in 30–100 µl water. A variety of special application protocols is
also available.
RNA purified with RNeasy technology has A260/280 ratios of
1.9–2.1 (measured in 10 mM Tris¬•Cl, pH 7.5) and is ideal for use in all
applications. Downstream applications include:
Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A+ RNA selection
More About This Kit
